Microprocessor UV-VIS Double Beam Spectrophotometer

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SALIENT FEATURES

Double beam optical system
Low noise and Low stray light
Large LCD display, can display curve
High quality grating, detector and lamps
Data and Curve can be stored in real-time
Auto setting WL, auto Blank
Lamps can be turned on/off individually
Easy to change Pri-aligned lamps
Reinforced baseboard and bracket assures durability

FUNCTIONS

Photometric: T%, Abs
Quantitative: Standard Curve
System Utility
WL Scan (Spectrum Scan)
Time Scan (Kinetics)
DNA/Protein Test

Basic Mode

To measure the Absorbance and transmittance

Quantitative

1. Coefficient Method
2. Standard Curve Up to 10 Standard sample may be used to establish a curve. Four methods for fitting a curve through the calibration points : Linear fit. Linear fit through zero, Square fit and cubic fit.

DNA/Protein Test

Concentration and DNA purity are quickly and easily calculated: Absorbance rations: 260 nm / 280 nm with optional subtracted absorbance at 320 nm. DNA concentration = 62.9XA260-36.0XA280 Protein concentration = 1552xA260- 757.3xA 280

Wavelength Scan

1. The wavelength scan intervals are 0.1,0.2,0.5,1,2,5 nm
2. High, Medium and low scan speed are available. They vary from 100 to 3600 nm/min
3. Wavelength are scanned from high to low so that the instrument waits at high WL. And it minimizes the degradation of UV sensitive samples.

Kinetics

Abs vs time graphs is displayed on the screen in real time wait time and measurement time up to 12 hours may be entered with time interval of 0.5,1,2,5,10,30 seconds and one min. Post-run manipulation includes
re-scalling, curve tracking and selection of the part of the curve required for rate calculation. Rate is calculated using a linear regression algorithm before multiplying be the entered factor.

 

 

SPECIFICATION

Wavelength Range                                          :               190-1100nm

Spectral Bandwidth                                         :               1nm

Optical System                                                  :               Double Beam, Blazed Holographic Grating (1200 lines/mm)

Wavelength Accuracy                                    :               ±0.5nm

Wavelength Repeatability                            :               ≤0.2nm

Wavelength Setting                                        :               Auto, Resolution 0.1nm

Photometric Range                                         :               0~200%T, -4~4A, 0~9999C

Photometric Accuracy                                    :               ±0.002 A (0~0.5A), ±0.003A (0.5~1A), ±0.3%T (0~100%T)

Photometric Repeatability                           :               ≤0.001 A (0~0.5A), ≤0.002A (0.5~1A), ≤0.2%T (0~100%T)

Stray Light                                                           :               ≤0.05%T(220/360nm)

Scan Speed                                                         :               High, Medium, Low. Max.3200 nm/minute

Baseline Flatness                                             :               ±0.0015A

Stability                                                                :               ±0.001A/h (500nm,0A)

Noise                                                               :                    ≤0.2%T/3min (250/500nm,0%T); ≤0.5%T/3min (250/500nm,100%T)

Sample Compartment                                   :               10mm Path length Cuvette

Detector                                                              :               Silicon Photodiode

Lamps                                                                   :               Tungsten Lamp & Deuterium Lamp (Pre-aligned)

Display                                                                  :               Graphic LCD (320*240 Dots)

Keypad                                                                 :               30-key Alphanumeric Membrance Keypad

Output Port                                                        :               USB Port

Printer                                                                  :               Mini Serial Printer; PC Printer

PC Software                                                       :               UV Analyst Scanning Software

Power Requirements                                     :               AC 90-250V, 50/60Hz

Dimension                                                          :               545x468x245mm

Weight                                                                 :               18kg

STANDARD CONFIGURATION

Glass Cell                       : 4 Nos.
Quartz cells                   : 2 Nos.
Instruments Cover      : 1 No.
Software CD                 : 1 N o.
USB Cable                    : 1 N o.
Operational Manual   : 1 N o .
Software Manual        : 1 N o .
Software key                : 1 N o.

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